Tissue Engineering

Tissue engineering has emerged as a potential strategy for tissue/organ repair and regeneration. This approach offers numerous advantages as it develops biodegradable implants with the necessary cues to promote bodily regeneration, and ultimately to form patient-derived tissues/organs. TEST Lab has made seminal contributions to the field of tissue engineering by developing and assessing graft options for bone and cartilage engineering in vitro as well as in animal models. Specifically, TEST Lab has developed oxygen-tension controlled matrices for large-area bone regeneration and polymer-gel structures to study stem cell chondrogenesis and hypertrophy, and eventual vascularized bone formation via endochondral ossification. Some of the current efforts include the development of advanced matrix systems with physical and structural cues to locally modulate cell behavior in tissue interfaces, specifically for bone-cartilage or osteochondral applications.

Related Publications:

Dorcemus D, Kim H and Nukavarapu SP. “Gradient Scaffold with Spatial Growth Factor Loading for Osteochondral Interface Engineering,” Biomedical Materials, 2021.

Dorcemus D, George E, Dealy C, Nukavarapu SP (2017) “Harnessing External Cues: Development and Evaluation of an In Vitroculture system for Osteochondral Tissue EngineeringTissue Eng Part A. 23, 719-737.

Mikael PE, Golebiowska AA, Xin X, Rowe DW, Nukavarapu SP. Evaluation of an Engineered Hybrid Matrix for Bone Regeneration via Endochondral Ossification. Ann Biomed Eng (2019).

Nukavarapu et al., Gradient Porous Scaffolds. US Patent# 9,707,322 B2 (2017).

Amini AR, Xu TO, Chidambaram R, Nukavarapu SPOxygen Tension Controlled Matrices with Osteogenic and Vasculogenic Cells for Vascularized Bone Regeneration In VivoTissue Eng Part A, 22, 610-620 (2016) (featured on cover)

Majumdar S, Pothirajan P, Dorcemus D, Nukavarapu SP, Kotecha M (2016) “High Field Sodium MRI Assessment of Stem Cell Chondrogenesis in a Tissue-Engineered MatrixAnn Biomed Eng., 44, 1120-1127.

Amini AR, Adams D, Laurencin CT, Nukavarapu SPOptimally Porous and Biomechanically Compatible Scaffolds for Large Area Bone RegenerationTissue Eng Part A., 18, 1376-1388 (2012).